Review



pet 32a expression vector  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    TaKaRa pet 32a expression vector
    Pet 32a Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 16196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+32a+expression+vector/EcoR+I/pmc11840546-84-17-26
    Average 99 stars, based on 16196 article reviews
    pet 32a expression vector - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: In Situ Growth of BiOI/MoS 2 Heterostructure as Pt Supports for Visible Light‐Assisted Electrocatalytic Methanol Oxidation Reaction
    Article Snippet: Aeromonas hydrophila, which is a major Gram‐negative bacterial pathogen in aquaculture, is responsible for many infectious dis‐ eases such as hemorrhagic septicaemia, hemorrhagic enteritis, ab‐ dominal dropsy, epizootic ulcerative syndrome, exophthalmos and red body disease (Dash, Sahoo, Gupta, Garg, & Dixit, 2014; Fang, Ge, & Sin, 2004).. The pathogen could infect a variety of fish, includ‐ ing carp (Mohanty, Mishra, Das, Jena, & Sahoo, 2008), channel cat‐ fish (Peatman et al., 2018), golden fish (Maji, Mali, & Joardar, 2006) and eel (Esteve & Alcaide, 2009), which results in huge economic losses to aquaculture industry around the world.. The application of antibiotics is presently not preferred, due to unavoidable drug resistance and side effects to the environment and human beings Received: 4 August 2018 | Revised: 2 August 2019 | Accepted: 3 August 2019 DOI: 10.1111/are.14311

    Article Title: Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida
    Article Snippet: .. The mature peptide sequences of lexA was amplified with primers (Supplemental materials 1) and cloned into the pET-32a(+) expression vector after digestion with EcoRI and XhoI (TaKaRa). ..

    Expressing:

    Article Title: In Situ Growth of BiOI/MoS 2 Heterostructure as Pt Supports for Visible Light‐Assisted Electrocatalytic Methanol Oxidation Reaction
    Article Snippet: Aeromonas hydrophila, which is a major Gram‐negative bacterial pathogen in aquaculture, is responsible for many infectious dis‐ eases such as hemorrhagic septicaemia, hemorrhagic enteritis, ab‐ dominal dropsy, epizootic ulcerative syndrome, exophthalmos and red body disease (Dash, Sahoo, Gupta, Garg, & Dixit, 2014; Fang, Ge, & Sin, 2004).. The pathogen could infect a variety of fish, includ‐ ing carp (Mohanty, Mishra, Das, Jena, & Sahoo, 2008), channel cat‐ fish (Peatman et al., 2018), golden fish (Maji, Mali, & Joardar, 2006) and eel (Esteve & Alcaide, 2009), which results in huge economic losses to aquaculture industry around the world.. The application of antibiotics is presently not preferred, due to unavoidable drug resistance and side effects to the environment and human beings Received: 4 August 2018 | Revised: 2 August 2019 | Accepted: 3 August 2019 DOI: 10.1111/are.14311

    Article Title: A novel hexamerin with an unexpected contribution to the prophenoloxidase activation system of the Chinese oak silkworm, Antheraea pernyi.
    Article Snippet: Funding information Support Plan of Shenyang Pharmaceutical University for Youth Development, Grant/Award Number: ZQN2014A06; Projects of Liaoning Provincial Education Department, Grant/Award Number: 2017LFW09; Natural Science Foundation of Liaoning Province, Grant/Award Number: 201700316; National Natural Science Foundation of China, Grant/Award Number: 31772518 Abstract Hexamerin was originally identified as a storage protein but later confirmed to be involved in many physiological processes.. In the present study, we cloned and characterized a novel hexamerin complementary DNA sequence from the Chinese oak silkworm, Antheraea pernyi (Ap‐hexamerin), which shows high homology with reported insect methionine‐ rich hexamerins.. The tissue distribution and time course of expression demonstrated that Ap‐hexamerin was predominantly synthesized in the fat body and the expression level was significantly increased in response to the microbial challenge, suggesting the relevance of Ap‐hexamerin to immune responses.

    Article Title: Functional Characterization of a New Degradation Peptide BmTX4-P1 from Traditional Chinese Scorpion Medicinal Material
    Article Snippet: .. The cDNA of BmTX4-P1 would be inserted into the pET-32a (+) expression vector by T4 DNA ligase (Takara, Japan). .. A QuikChange Site-Directed Mutagenesis Kit (Stratagene, CA, USA) was applied to obtain the mutant plasmids based on the recombinant plasmids of BmTX4-P1.

    Article Title: Plasminogen activator inhibitor-1 regulates cell adhesion by binding to the somatomedin B domain of vitronectin.
    Article Snippet: Plasminogen activator inhibitor-1 (PAI-1) binds to the somatomedin B (SMB) domain of vitronectin.. It inhibits the adhesion of U937 cells to vitronectin by competing with the urokinase receptor (uPAR; CD87) on these cells for binding to the same domain.. Although the inhibitor also blocks integrin-mediated cell adhesion, the molecular basis of this effect is unclear.

    Article Title: Functional Characterization of a New Degradation Peptide BmTX4-P1 from Traditional Chinese Scorpion Medicinal Material.
    Article Snippet: .. The cDNA of BmTX4-P1 would be inserted into the pET-32a (+) expression vector by T4 DNA ligase (Takara, Japan). .. A QuikChange Site-Directed Mutagenesis Kit (Stratagene, CA, USA) was applied to obtain the mutant plasmids based on the recombinant plasmids of BmTX4-P1.

    Article Title: Molecular characterization and tissue localization of glutathione <i>S</i>-transferase from adult <i>Ancylostoma ceylanicum</i>
    Article Snippet: Glutathione S-transferases (GSTs) are a detoxifying enzyme family that is essential for parasite blood-feeding and survival, and represent potential targets for hookworm vaccine development.. Multiple GST-encoding complementary DNAs (cDNAs) have been cloned from Ancylostoma caninum and Necator americanus, but there are no reports about the cloning of this enzyme from Ancylostoma ceylanicum, the animal-derived zoonotic hookworm.. To study the molecular nature and tissue localization of GST of A. ceylanicum (AceGST), we designed primers based on the GST gene sequence of A. ceylanicum in GenBank, amplified the Ace-GST cDNA by reverse transcription polymerase chain reaction, and analysed its homology and genetic evolution relationship.

    Article Title: Goose surfactant protein A inhibits the growth of avian pathogenic Escherichia coli via an aggregation-dependent mechanism that decreases motility and increases membrane permeability.
    Article Snippet: Pulmonary collectins have been reported to bind carbohydrates on pathogens and inhibit infection by agglutination, neutralization, and opsonization.. In this study, surfactant protein A (SP-A) was identified from goose lung and characterized at expressionand agglutination-functional levels.. The deduced amino acid sequence of goose surfactant protein A (gSP-A) has two characteristic structures: a shorter, collagen-like region and a carbohydrate recognition domain.

    Article Title: Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida
    Article Snippet: .. The mature peptide sequences of lexA was amplified with primers (Supplemental materials 1) and cloned into the pET-32a(+) expression vector after digestion with EcoRI and XhoI (TaKaRa). ..

    Plasmid Preparation:

    Article Title: In Situ Growth of BiOI/MoS 2 Heterostructure as Pt Supports for Visible Light‐Assisted Electrocatalytic Methanol Oxidation Reaction
    Article Snippet: Aeromonas hydrophila, which is a major Gram‐negative bacterial pathogen in aquaculture, is responsible for many infectious dis‐ eases such as hemorrhagic septicaemia, hemorrhagic enteritis, ab‐ dominal dropsy, epizootic ulcerative syndrome, exophthalmos and red body disease (Dash, Sahoo, Gupta, Garg, & Dixit, 2014; Fang, Ge, & Sin, 2004).. The pathogen could infect a variety of fish, includ‐ ing carp (Mohanty, Mishra, Das, Jena, & Sahoo, 2008), channel cat‐ fish (Peatman et al., 2018), golden fish (Maji, Mali, & Joardar, 2006) and eel (Esteve & Alcaide, 2009), which results in huge economic losses to aquaculture industry around the world.. The application of antibiotics is presently not preferred, due to unavoidable drug resistance and side effects to the environment and human beings Received: 4 August 2018 | Revised: 2 August 2019 | Accepted: 3 August 2019 DOI: 10.1111/are.14311

    Article Title: Molecular characterization and tissue localization of glutathione <i>S</i>-transferase from adult <i>Ancylostoma ceylanicum</i>
    Article Snippet: Glutathione S-transferases (GSTs) are a detoxifying enzyme family that is essential for parasite blood-feeding and survival, and represent potential targets for hookworm vaccine development.. Multiple GST-encoding complementary DNAs (cDNAs) have been cloned from Ancylostoma caninum and Necator americanus, but there are no reports about the cloning of this enzyme from Ancylostoma ceylanicum, the animal-derived zoonotic hookworm.. To study the molecular nature and tissue localization of GST of A. ceylanicum (AceGST), we designed primers based on the GST gene sequence of A. ceylanicum in GenBank, amplified the Ace-GST cDNA by reverse transcription polymerase chain reaction, and analysed its homology and genetic evolution relationship.

    Transformation Assay:

    Article Title: In Situ Growth of BiOI/MoS 2 Heterostructure as Pt Supports for Visible Light‐Assisted Electrocatalytic Methanol Oxidation Reaction
    Article Snippet: Aeromonas hydrophila, which is a major Gram‐negative bacterial pathogen in aquaculture, is responsible for many infectious dis‐ eases such as hemorrhagic septicaemia, hemorrhagic enteritis, ab‐ dominal dropsy, epizootic ulcerative syndrome, exophthalmos and red body disease (Dash, Sahoo, Gupta, Garg, & Dixit, 2014; Fang, Ge, & Sin, 2004).. The pathogen could infect a variety of fish, includ‐ ing carp (Mohanty, Mishra, Das, Jena, & Sahoo, 2008), channel cat‐ fish (Peatman et al., 2018), golden fish (Maji, Mali, & Joardar, 2006) and eel (Esteve & Alcaide, 2009), which results in huge economic losses to aquaculture industry around the world.. The application of antibiotics is presently not preferred, due to unavoidable drug resistance and side effects to the environment and human beings Received: 4 August 2018 | Revised: 2 August 2019 | Accepted: 3 August 2019 DOI: 10.1111/are.14311

    Article Title: A novel hexamerin with an unexpected contribution to the prophenoloxidase activation system of the Chinese oak silkworm, Antheraea pernyi.
    Article Snippet: Funding information Support Plan of Shenyang Pharmaceutical University for Youth Development, Grant/Award Number: ZQN2014A06; Projects of Liaoning Provincial Education Department, Grant/Award Number: 2017LFW09; Natural Science Foundation of Liaoning Province, Grant/Award Number: 201700316; National Natural Science Foundation of China, Grant/Award Number: 31772518 Abstract Hexamerin was originally identified as a storage protein but later confirmed to be involved in many physiological processes.. In the present study, we cloned and characterized a novel hexamerin complementary DNA sequence from the Chinese oak silkworm, Antheraea pernyi (Ap‐hexamerin), which shows high homology with reported insect methionine‐ rich hexamerins.. The tissue distribution and time course of expression demonstrated that Ap‐hexamerin was predominantly synthesized in the fat body and the expression level was significantly increased in response to the microbial challenge, suggesting the relevance of Ap‐hexamerin to immune responses.

    Purification:

    Article Title: Molecular characterization and tissue localization of glutathione <i>S</i>-transferase from adult <i>Ancylostoma ceylanicum</i>
    Article Snippet: Glutathione S-transferases (GSTs) are a detoxifying enzyme family that is essential for parasite blood-feeding and survival, and represent potential targets for hookworm vaccine development.. Multiple GST-encoding complementary DNAs (cDNAs) have been cloned from Ancylostoma caninum and Necator americanus, but there are no reports about the cloning of this enzyme from Ancylostoma ceylanicum, the animal-derived zoonotic hookworm.. To study the molecular nature and tissue localization of GST of A. ceylanicum (AceGST), we designed primers based on the GST gene sequence of A. ceylanicum in GenBank, amplified the Ace-GST cDNA by reverse transcription polymerase chain reaction, and analysed its homology and genetic evolution relationship.

    Recombinant:

    Article Title: Molecular characterization and tissue localization of glutathione <i>S</i>-transferase from adult <i>Ancylostoma ceylanicum</i>
    Article Snippet: Glutathione S-transferases (GSTs) are a detoxifying enzyme family that is essential for parasite blood-feeding and survival, and represent potential targets for hookworm vaccine development.. Multiple GST-encoding complementary DNAs (cDNAs) have been cloned from Ancylostoma caninum and Necator americanus, but there are no reports about the cloning of this enzyme from Ancylostoma ceylanicum, the animal-derived zoonotic hookworm.. To study the molecular nature and tissue localization of GST of A. ceylanicum (AceGST), we designed primers based on the GST gene sequence of A. ceylanicum in GenBank, amplified the Ace-GST cDNA by reverse transcription polymerase chain reaction, and analysed its homology and genetic evolution relationship.

    Polymerase Chain Reaction:

    Article Title: Goose surfactant protein A inhibits the growth of avian pathogenic Escherichia coli via an aggregation-dependent mechanism that decreases motility and increases membrane permeability.
    Article Snippet: Pulmonary collectins have been reported to bind carbohydrates on pathogens and inhibit infection by agglutination, neutralization, and opsonization.. In this study, surfactant protein A (SP-A) was identified from goose lung and characterized at expressionand agglutination-functional levels.. The deduced amino acid sequence of goose surfactant protein A (gSP-A) has two characteristic structures: a shorter, collagen-like region and a carbohydrate recognition domain.

    Construct:

    Article Title: Goose surfactant protein A inhibits the growth of avian pathogenic Escherichia coli via an aggregation-dependent mechanism that decreases motility and increases membrane permeability.
    Article Snippet: Pulmonary collectins have been reported to bind carbohydrates on pathogens and inhibit infection by agglutination, neutralization, and opsonization.. In this study, surfactant protein A (SP-A) was identified from goose lung and characterized at expressionand agglutination-functional levels.. The deduced amino acid sequence of goose surfactant protein A (gSP-A) has two characteristic structures: a shorter, collagen-like region and a carbohydrate recognition domain.

    Amplification:

    Article Title: Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida
    Article Snippet: .. The mature peptide sequences of lexA was amplified with primers (Supplemental materials 1) and cloned into the pET-32a(+) expression vector after digestion with EcoRI and XhoI (TaKaRa). ..



    Similar Products

    99
    TaKaRa pet 32a expression vector
    Pet 32a Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+32a+expression+vector/EcoR+I/pmc11840546-84-17-26
    Average 99 stars, based on 1 article reviews
    pet 32a expression vector - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    90
    Millipore pet-32a expression vector
    Pet 32a Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+32a+expression+vector/pet28a/pm39187065-51-11-14
    Average 90 stars, based on 1 article reviews
    pet-32a expression vector - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Millipore the pet-32a (+) expression vector
    The Pet 32a (+) Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+32a+expression+vector/pet28a/pmc11299062-109-23-27
    Average 90 stars, based on 1 article reviews
    the pet-32a (+) expression vector - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Millipore pet-32a( +) expression vector
    Pet 32a( +) Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+32a+expression+vector/pet28a/pm38974359-53-9-13
    Average 90 stars, based on 1 article reviews
    pet-32a( +) expression vector - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    93
    Addgene inc pet32a expression vector
    Pet32a Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+32a+expression+vector/pET-32a(%2B)-NRG1B1+(Plasmid+%23120287)/pm38191049-92-4-7
    Average 93 stars, based on 1 article reviews
    pet32a expression vector - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    90
    tiangen biotech co pet-32a expression vector
    Pet 32a Expression Vector, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+32a+expression+vector/pet+32a/10__2139_slash_ssrn__4721125-92-6-18
    Average 90 stars, based on 1 article reviews
    pet-32a expression vector - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Millipore prokaryotic expression vector pet-32a
    Endonuclease digestion of the expression plasmid. M: DNA marker DL10000. Lane 1: the product of eukaryotic expression plasmid pVAX1-14EGT digested by KpnI and XhoI restriction enzymes. Lane 2: the product of the <t>prokaryotic</t> expression plasmid <t>pET-32a-14EGT</t> digested by KpnI and XhoI restriction enzymes.
    Prokaryotic Expression Vector Pet 32a, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+32a+expression+vector/pet28a/pmc10720236-116-7-14
    Average 90 stars, based on 1 article reviews
    prokaryotic expression vector pet-32a - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Millipore pet-32a(+) expression vector
    Endonuclease digestion of the expression plasmid. M: DNA marker DL10000. Lane 1: the product of eukaryotic expression plasmid pVAX1-14EGT digested by KpnI and XhoI restriction enzymes. Lane 2: the product of the <t>prokaryotic</t> expression plasmid <t>pET-32a-14EGT</t> digested by KpnI and XhoI restriction enzymes.
    Pet 32a(+) Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+32a+expression+vector/pet28a/pm37368679-362-7-10
    Average 90 stars, based on 1 article reviews
    pet-32a(+) expression vector - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    Image Search Results


    Endonuclease digestion of the expression plasmid. M: DNA marker DL10000. Lane 1: the product of eukaryotic expression plasmid pVAX1-14EGT digested by KpnI and XhoI restriction enzymes. Lane 2: the product of the prokaryotic expression plasmid pET-32a-14EGT digested by KpnI and XhoI restriction enzymes.

    Journal: Veterinary Research

    Article Title: Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens

    doi: 10.1186/s13567-023-01253-y

    Figure Lengend Snippet: Endonuclease digestion of the expression plasmid. M: DNA marker DL10000. Lane 1: the product of eukaryotic expression plasmid pVAX1-14EGT digested by KpnI and XhoI restriction enzymes. Lane 2: the product of the prokaryotic expression plasmid pET-32a-14EGT digested by KpnI and XhoI restriction enzymes.

    Article Snippet: The eukaryotic expression vector pVAX1 and the prokaryotic expression vector pET-32a were purchased from Novagen (Darmstadt, Germany).

    Techniques: Expressing, Plasmid Preparation, Marker